RNA-seq of B. subtilis: DdhbA monoculture - SRA

SRX20625958: RNA-seq of B. subtilis: DdhbA monoculture
1 ILLUMINA (Illumina NovaSeq 6000) run: 9.3M spots, 2.8G bases, 877.2Mb downloads

Design: First, the ribosomal RNAs from both eukaryotes and prokaryotes were depleted from total RNA samples. The remained RNAs were fragmented into around 250~300 bp and then reverse-transcribed into double-stranded cDNAs which subsequently went through end repair, A tailing and adapter ligation. After fragments size selection and PCR amplification, the metatranscriptome library was ready for library QC and sequencing. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.

Submitted by: Technical university of Denmark

Study: Competition for iron shapes metabolic antagonism between Bacillus sub-tilis and Pseudomonas

PRJNA956831 • SRP433980 • All experiments • All runs

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Assessing the mechanism of antagonism between Bacillus subtilis NCIB 3610 and a Pseudomonas marginalis soil isolate

Sample: DdhbA monoculture

SAMN35564847 • SRS17925589 • All experiments • All runs

Organism: Bacillus subtilis

Library:

Name: d1

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq